ISO 11063:2020
ISO 11063:2020 Soil quality – Direct extraction of soil DNA
CDN $124.00
Description
The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.
Edition
2
Published Date
2020-09-29
Status
PUBLISHED
Pages
10
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 32210:2022 Sustainable finance – Guidance on the application of sustainability principles for organizations in the financial sector
CDN $251.00 Add to cart -
ISO 23434:2021 Industrial trucks – Sustainability – Part 1: Vocabulary
CDN $82.00 Add to cart -
ISO 41011:2024 Facility management – Vocabulary
CDN $251.00 Add to cart -
ISO 20480:2021 Fine bubble technology – General principles for usage and measurement of fine bubbles – Part 3: Methods for generating fine bubbles
CDN $186.00 Add to cart
