ISO 14461:2005
ISO 14461:2005 Milk and milk products – Quality control in microbiological laboratories – Part 2: Determination of the reliability of colony counts of parallel plates and subsequent dilution steps
CDN $186.00
Description
ISO 14461-2|IDF 169-2:2005 describes a routine procedure for the evaluation of results of the enumeration of microorganisms using colony-count methods with subsequent 10-fold dilution steps and one plate or two parallel plates within each dilution step.
This routine procedure is applied regularly in each laboratory performing colony counts. It provides criteria for the acceptability of differences between the results from parallel plates and subsequent dilution steps, as follows.
The results (colony counts) obtained from parallel plates are compared with tabulated limits for given colony counts. If these limits are exceeded, a technical problem when performing the parallel determinations may be indicated.
The results (sums of colony counts) of two parallel plates of two subsequent 10-fold dilution steps are compared with tabulated limits for given sums of colony counts. If these limits are exceeded, a technical problem when performing the dilutions may be indicated.
If the limits mentioned above are exceeded in more cases than expected, this indicates that the test procedure lacks reliability.
Edition
1
Published Date
2005-04-12
Status
PUBLISHED
Pages
18
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
ISO 14461-2|IDF 169-2:2005 describes a routine procedure for the evaluation of results of the enumeration of microorganisms using colony-count methods with subsequent 10-fold dilution steps and one plate or two parallel plates within each dilution step.
This routine procedure is applied regularly in each laboratory performing colony counts. It provides criteria for the acceptability of differences between the results from parallel plates and subsequent dilution steps, as follows.
The results (colony counts) obtained from parallel plates are compared with tabulated limits for given colony counts. If these limits are exceeded, a technical problem when performing the parallel determinations may be indicated.
The results (sums of colony counts) of two parallel plates of two subsequent 10-fold dilution steps are compared with tabulated limits for given sums of colony counts. If these limits are exceeded, a technical problem when performing the dilutions may be indicated.
If the limits mentioned above are exceeded in more cases than expected, this indicates that the test procedure lacks reliability.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 8196:2022 Milk – Definition and evaluation of the overall accuracy of alternative methods of milk analysis – Part 3: Protocol for the evaluation and validation of alternative quantitative methods of milk analysis
CDN $336.00 Add to cart -
ISO 4214:2022 Milk and milk products – Determination of amino acids in infant and adult/paediatric nutritional formulas and dairy products
CDN $295.00 Add to cart -
ISO 20128:2006 Milk products – Enumeration of presumptive Lactobacillus acidophilus on a selective medium – Colony-count technique at 37 degrees C
CDN $124.00 Add to cart -
ISO 14673:2004 Milk and milk products – Determination of nitrate and nitrite contents – Part 2: Method using segmented flow analysis (Routine method)
CDN $186.00 Add to cart
