ISO 15216:2017
ISO 15216:2017 Microbiology of the food chain – Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Part 1: Method for quantification
CDN $347.00
Description
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
Edition
1
Published Date
2017-03-09
Status
PUBLISHED
Pages
48
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 17468:2023 Microbiology of the food chain – Technical requirements and guidance on the establishment or revision of a standardized reference method
CDN $192.00 Add to cart -
ISO 15213:2024 Microbiology of the food chain – Horizontal method for the detection and enumeration of Clostridium spp. – Part 3: Detection of Clostridium perfringens
CDN $305.00 Add to cart -
ISO 10272:2023 Microbiology of the food chain – Horizontal method for detection and enumeration of Campylobacter spp. – Part 1: Detection method – Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp., the use of growth supplement in Preston broth and changes in the performance testing of culture media
CDN $258.00 Add to cart -
ISO 23418:2022 Microbiology of the food chain – Whole genome sequencing for typing and genomic characterization of bacteria – General requirements and guidance
CDN $347.00 Add to cart
