ISO 20224:2020
ISO 20224:2020 Molecular biomarker analysis – Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR – Part 7: Donkey DNA detection method
CDN $195.00
Description
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of donkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of donkey material derived from donkey (Equus asinus), mule (Equus caballus ‚ôÄ √ó Equus asinus ‚ôÇ) and hinny (Equus caballus ‚ôÇ √ó Equus asinus ‚ôÄ). The assay also detects the species zebra (Equus burchellii).
The target sequence is a partial fragment of the Equus asinus isolate Maral har breed Guanzhong donkey unplaced genomic scaffold, ASM130575v1 scaffold786, whole genome shotgun sequence (i.e. GenBank accession number NW_014638576.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ‚â• 95 % replicability at this concentration (LOD95 %).
Edition
1
Published Date
2020-07-31
Status
PUBLISHED
Pages
14
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of donkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of donkey material derived from donkey (Equus asinus), mule (Equus caballus ‚ôÄ √ó Equus asinus ‚ôÇ) and hinny (Equus caballus ‚ôÇ √ó Equus asinus ‚ôÄ). The assay also detects the species zebra (Equus burchellii).
The target sequence is a partial fragment of the Equus asinus isolate Maral har breed Guanzhong donkey unplaced genomic scaffold, ASM130575v1 scaffold786, whole genome shotgun sequence (i.e. GenBank accession number NW_014638576.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ‚â• 95 % replicability at this concentration (LOD95 %).
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 32210:2022 Sustainable finance – Guidance on the application of sustainability principles for organizations in the financial sector
CDN $263.00 Add to cart -
ISO 6707:2017 Buildings and civil engineering works – Vocabulary – Part 2: Contract and communication terms
CDN $352.00 Add to cart -
ISO 1990:1982 Fruits – Nomenclature – First list
CDN $131.00 Add to cart -
ISO 1991:1982 Vegetables – Nomenclature – First list
CDN $131.00 Add to cart
