ISO 21569:2016
ISO 21569:2016 Horizontal methods for molecular biomarker analysis – Methods of analysis for the detection of genetically modified organisms and derived products – Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence
CDN $130.00
Description
ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 base pairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GM plants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).
The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may be suitable also for analysis of other products such as feedstuffs and seeds. The procedure requires the extraction of an adequate quantity and quality of amplifiable DNA from the test sample.
The DNA sequence amplified by the P-FMV element-specific method can be detected in samples which contain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.
Edition
1
Published Date
2016-11-02
Status
PUBLISHED
Pages
10
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 base pairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GM plants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).
The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may be suitable also for analysis of other products such as feedstuffs and seeds. The procedure requires the extraction of an adequate quantity and quality of amplifiable DNA from the test sample.
The DNA sequence amplified by the P-FMV element-specific method can be detected in samples which contain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 1990:1982 Fruits – Nomenclature – First list
CDN $130.00 Add to cart -
ISO 21276:2018 Clean cookstoves and clean cooking solutions – Vocabulary
CDN $85.00 Add to cart -
ISO 6707:2017 Buildings and civil engineering works – Vocabulary – Part 2: Contract and communication terms
CDN $349.00 Add to cart -
ISO 6405:2017 Earth-moving machinery – Symbols for operator controls and other displays – Part 2: Symbols for specific machines, equipment and accessories
CDN $393.00 Add to cart
