Your cart is currently empty!
ISO 21569:2021
ISO 21569:2021 Molecular biomarker analysis – Methods of analysis for the detection of genetically modified organisms and derived products – Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products
CDN $115.00
Description
This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.
Edition
2
Published Date
2021-07-05
Status
PUBLISHED
Pages
10
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 20224:2022 Molecular biomarker analysis – Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR – Part 8: Turkey DNA detection method
CDN $173.00 Add to cart -
ISO 20224:2020 Molecular biomarker analysis – Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR – Part 6: Horse DNA detection method
CDN $173.00 Add to cart -
ISO 21569:2020 Horizontal methods for molecular biomarker analysis – Methods of analysis for the detection of genetically modified organisms and derived products – Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms
CDN $173.00 Add to cart -
ISO 16578:2022 Molecular biomarker analysis – Requirements for microarray detection of specific nucleic acid sequences
CDN $173.00 Add to cart
