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ISO 23418:2022
ISO 23418:2022 Microbiology of the food chain – Whole genome sequencing for typing and genomic characterization of bacteria – General requirements and guidance
CDN $312.00
Description
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
-    products intended for human consumption;
-    products intended for animal feed;
-    environmental samples from food and feed handling and production areas;
-    samples from the primary production stage.
Edition
1
Published Date
2022-06-09
Status
PUBLISHED
Pages
45
Language
English
Format
Secure PDF
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Abstract
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
-    products intended for human consumption;
-    products intended for animal feed;
-    environmental samples from food and feed handling and production areas;
-    samples from the primary production stage.
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