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“ISO 10272:2023 Microbiology of the food chain – Horizontal method for detection and enumeration of Campylobacter spp. – Part 2: Colony-count technique – Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and changes in the performance testing of culture media” has been added to your cart. View cart
ISO 23418:2022
ISO 23418:2022 Microbiology of the food chain – Whole genome sequencing for typing and genomic characterization of bacteria – General requirements and guidance
CDN $312.00
Description
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
-    products intended for human consumption;
-    products intended for animal feed;
-    environmental samples from food and feed handling and production areas;
-    samples from the primary production stage.
Edition
1
Published Date
2022-06-09
Status
PUBLISHED
Pages
45
Language
English
Format
Secure PDF
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Abstract
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
-    products intended for human consumption;
-    products intended for animal feed;
-    environmental samples from food and feed handling and production areas;
-    samples from the primary production stage.
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