Your cart is currently empty!
“ISO 23036:2021 Microbiology of the food chain – Methods for the detection of Anisakidae L3 larvae in fish and fishery products – Part 2: Artificial digestion method” has been added to your cart. View cart
ISO 23418:2022
ISO 23418:2022 Microbiology of the food chain – Whole genome sequencing for typing and genomic characterization of bacteria – General requirements and guidance
CDN $312.00
Description
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
-    products intended for human consumption;
-    products intended for animal feed;
-    environmental samples from food and feed handling and production areas;
-    samples from the primary production stage.
Edition
1
Published Date
2022-06-09
Status
PUBLISHED
Pages
45
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
-    products intended for human consumption;
-    products intended for animal feed;
-    environmental samples from food and feed handling and production areas;
-    samples from the primary production stage.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 15213:2023 Microbiology of the food chain – Horizontal method for the detection and enumeration of Clostridium spp. – Part 1: Enumeration of sulfite-reducing Clostridium spp. by colony-count technique
CDN $233.00 Add to cart -
ISO 20976:2022 Microbiology of the food chain – Requirements and guidelines for conducting challenge tests of food and feed products – Part 2: Challenge tests to study inactivation potential and kinetic parameters
CDN $233.00 Add to cart -
ISO 20836:2021 Microbiology of the food chain – Polymerase chain reaction (PCR) for the detection of microorganisms – Thermal performance testing of thermal cyclers
CDN $273.00 Add to cart -
ISO 16140:2024 Microbiology of the food chain – Method validation – Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method – Amendment 1: Revision of qualitative method comparison study data evaluation, relative level of detection calculations in the interlaboratory study, calculation and interpretation of the relative trueness study, and inclusion of a commercial sterility testing protocol for specific products
CDN $233.00 Add to cart
