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ISO 6579:2012

ISO 6579:2012 Microbiology of food and animal feed – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 2: Enumeration by a miniaturized most probable number technique

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Description

ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN).

The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae.

It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi.

The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).

Edition

1

Published Date

2012-11-05

Status

PUBLISHED

Pages

18

Language Detail Icon

English

Format Secure Icon

Secure PDF

Abstract

ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN).

The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae.

It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi.

The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).

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