ISO 20948:2024

Description

This document specifies a method for the determination of the aflatoxins B₁, B₂, G₁ and G₂ in vegetable fats and oils, including peanut oil, sesame oil, olive oil, corn oil, sunflower oil, rapeseed oil and coconut oil, using immunoaffinity column clean-up and high-performance liquid chromatography with post-column derivatization.

The limits of quantification for the aflatoxins B₁, B₂, G₁ and G₂, and for the sum of aflatoxins B₁, B₂, G₁ and G₂, are 1¬†Œºg/kg, 0,25¬†Œºg/kg, 0,5¬†Œºg/kg, 0,25¬†Œºg/kg and 1¬†Œºg/kg, respectively.

The validation was carried out over the following concentration ranges:

–¬†¬†¬†¬† aflatoxin B₁¬†=¬†1¬†Œºg/kg to 20¬†Œºg/kg;

–¬†¬†¬†¬† total aflatoxins¬†=¬†2¬†Œºg/kg to 52¬†Œºg/kg.