ISO 19040:2018
ISO 19040:2018 Water quality – Determination of the estrogenic potential of water and waste water – Part 3: In vitro human cell-based reporter gene assay
CDN $349.00
Description
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha.
This method is applicable to:
– fresh water;
– waste water;
– aqueous extracts and leachates;
– eluates of sediments (fresh water);
– pore water;
– aqueous solutions of single substances or of chemical mixtures;
– drinking water;
Рthe limit of quantification (LOQ) of this method for the direct analysis of water samples is between 0,3 ng/l and 1 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper working range was evaluated [based on the results of the international interlaboratory trial (see Table F.3)] up to a level of 75 ng EEQ/l. Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre concentration of water samples can prove necessary if their estrogenic potential is below the given LOQ.
Edition
1
Published Date
2018-08-07
Status
PUBLISHED
Pages
40
Language
English
Format
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha.
This method is applicable to:
- fresh water;
- waste water;
- aqueous extracts and leachates;
- eluates of sediments (fresh water);
- pore water;
- aqueous solutions of single substances or of chemical mixtures;
- drinking water;
- the limit of quantification (LOQ) of this method for the direct analysis of water samples is between 0,3 ng/l and 1 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper working range was evaluated [based on the results of the international interlaboratory trial (see Table F.3)] up to a level of 75 ng EEQ/l. Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre concentration of water samples can prove necessary if their estrogenic potential is below the given LOQ.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-
ISO 16665:2014 Water quality – Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna
CDN $307.00 Add to cart -
ISO 19827:2016 Water quality – Determination of the acute toxicity to the freshwater rotifer Brachionus calyciflorus
CDN $193.00 Add to cart -
ISO 17994:2014 Water quality – Requirements for the comparison of the relative recovery of microorganisms by two quantitative methods
CDN $261.00 Add to cart -
ISO 17244:2015 Water quality – Determination of the toxicity of water samples on the embryo-larval development of Japanese oyster (Crassostrea gigas) and mussel (Mytilus edulis or Mytilus galloprovincialis)
CDN $251.00 Add to cart
