
ISO 12966:2017
ISO 12966:2017 Animal and vegetable fats and oils – Gas chromatography of fatty acid methyl esters – Part 2: Preparation of methyl esters of fatty acids
CDN $195.00
Description
ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely:
a) a “rapid” transmethylation procedure under alkaline conditions;
b) a “general” transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
Edition
2
Published Date
2017-03-31
Status
PUBLISHED
Pages
15
Format 
Secure PDF
Secure – PDF details
- Save your file locally or view it via a web viewer
- Viewing permissions are restricted exclusively to the purchaser
- Device limits - 3
- Printing – Enabled only to print (1) copy
See more about our Environmental Commitment
Abstract
ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely:
a) a "rapid" transmethylation procedure under alkaline conditions;
b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
Previous Editions
Can’t find what you are looking for?
Please contact us at:
Related Documents
-

ISO 18395:2005 Animal and vegetable fats and oils – Determination of monoacylglycerols, diacylglycerols, triacylglycerols and glycerol by high-performance size-exclusion chromatography (HPSEC)
CDN $131.00 Add to cart -

ISO 20948:2024 Vegetable fats and oils – Determination of aflatoxins B1, B2, G1 and G2 by immunoaffinity column clean-up and high-performance liquid chromatography
CDN $263.00 Add to cart -

ISO 18363:2025 Animal and vegetable fats and oils – Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS – Part 2: Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol
CDN $263.00 Add to cart -

ISO 13884:2003 Animal and vegetable fats and oils – Determination of isolated trans isomers by infrared spectrometry
CDN $131.00 Add to cart







