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ISO 23719:2025

ISO 23719:2025 Cereals and cereal products — Determination of 17 mycotoxins by ultra-high-performance liquid chromatography and tandem mass spectrometry method (UHPLC-MS/MS)

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This publication was last reviewed and confirmed in 2025.

Cereals and cereal products — Determination of 17 mycotoxins by ultra-high-performance liquid chromatography and tandem mass spectrometry method (UHPLC-MS/MS)

Description

This document specifies a method for the quantitative determination of 17 mycotoxins in cereals and cereal products (e.g. wheat, maize, husked rice, rice and their products) using ultra-high-performance liquid chromatography and tandem mass spectrometry method (UHPLC-MS/MS).

The 17 mycotoxins are aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, deoxynivalenol, nivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, zearalenone, ochratoxin A, fumonisin B1, fumonisin B2, fumonisin B3, T-2 toxin, HT-2 toxin and sterigmatocystin.

This document does not apply to foods for infants and young children.

This document is applicable to other products (e.g. nuts) provided that the method is validated for each individual case.

The calibration range of the method and the validated range during the interlaboratory study for each mycotoxin are listed in Table A.1.

Edition

1

Published Date

2026-06-18

Status

PUBLISHED

Pages

40

Language Detail Icon

English

Format Secure Icon

Secure PDF

Abstract

This document specifies a method for the quantitative determination of 17 mycotoxins in cereals and cereal products (e.g. wheat, maize, husked rice, rice and their products) using ultra-high-performance liquid chromatography and tandem mass spectrometry method (UHPLC-MS/MS).

The 17 mycotoxins are aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, deoxynivalenol, nivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, zearalenone, ochratoxin A, fumonisin B1, fumonisin B2, fumonisin B3, T-2 toxin, HT-2 toxin and sterigmatocystin.

This document does not apply to foods for infants and young children.

This document is applicable to other products (e.g. nuts) provided that the method is validated for each individual case.

The calibration range of the method and the validated range during the interlaboratory study for each mycotoxin are listed in Table A.1.

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